Modulating Immunogenicity and Reactogenicity in mRNA-Lipid Nanoparticle Vaccines through Lipid Component Optimization.

ABSTRACT: Messenger RNA (mRNA) vaccines effectively induce antibody production and T cell responses. However, adverse reactions, such as fatigue and fever, following administration remain a key challenge. To modulate the immunogenicity and reactogenicity of mRNA vaccines, the optimization of lipid nanoparticle (LNP) formulations has been attempted, particularly by screening ionizable lipids. In contrast, the potential impact of modifying other LNP components─poly(ethylene glycol) (PEG)-lipids, cholesterol, and phospholipids─on overall vaccine effects and adverse reactions remains underexplored. Here, we prepared mRNA-LNP formulations with altered structures and molar ratios of these components to assess their effects on in vivo protein expression, as well as on the induction of antigen-specif

SUBMITTER: Kawaguchi Y 

PROVIDER: S-EPMC12333428 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature