Tailoring of Prematurely Released Polyketide Intermediates in Piericidin Biosynthesis.

ABSTRACT: Piericidins make up a group of microbial-derived polyketide natural products with a wide variety of biological activities. Their biosynthetic gene clusters (BGCs) have been identified in several strains of Streptomyces. In Streptomyces pactum, the piericidin BGC splits into two loci consisting of genes that encode six modular (type I) polyketide synthases and five post polyketide synthase (PKS) modification enzymes. Here, we report that inactivation of the pieA5 gene of the piericidin PKS in a mutant strain of S. pactum, in which several other major biosynthetic pathways have been inactivated, led to the identification of four piericidin polyketide intermediates (1-4) that were offloaded prematurely from the PKS. Compounds 1-3 are der

SUBMITTER: Alharbi HA 

PROVIDER: S-EPMC12333601 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature