Chronic Obstructive Pulmonary Disease Airway Epithelial Cell-derived Extracellular Vesicles Spread Cellular Senescence via MicroRNA-34a.

ABSTRACT: Chronic obstructive pulmonary disease (COPD) is associated with the acceleration of lung aging and the accumulation of senescent cells in lung tissue. MicroRNA-34a (miR-34a) induces senescence by suppressing the antiaging molecule sirtuin-1 (SIRT1). Senescent cells spread senescence to neighboring and distant cells, favoring COPD progression and its comorbidities. Mechanisms for spreading senescence remain undetermined but may be mediated by the transfer of microRNAs in extracellular vesicles (EVs). We analyzed the microRNA content of EVs in COPD and explored their effect on cellular senescence of healthy cells. EVs were isolated from small airway epithelial cells (SAECs) from healthy donors or patients with COPD. Recipient healthy SAECs were cultured with EVs, and the expression of miR-34

SUBMITTER: Devulder JV 

PROVIDER: S-EPMC12334839 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature