Characterization of a Single-Molecule Sensitive Digital Flow Cytometer for Amplification-Free Digital Assays.

ABSTRACT: Digital assays such as digital PCR for nucleic acids and digital ELISA for proteins provide absolute quantitation and greater accuracy, sensitivity, and reproducibility than their analogue counterparts (real-time PCR and standard ELISA), but current digital assays involve amplification (e.g., DNA amplification in digital PCR and signal amplification in digital ELISA), which makes high multiplexing difficult, often requires complex and expensive sample compartmentalization, and adds reaction steps. We have developed a single-molecule sensitive flow cytometer, which we termed a digital flow cytometer (dFC). dFC optimizes the sensitivity and efficiency of single-molecule detection by using smaller, planar microfluidic channels, a smaller probe volume, and a shorter working distance/higher num

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC12335221 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature