MSstatsTMT Improves Accuracy of Thermal Proteome Profiling.

ABSTRACT: Thermal proteome profiling investigates protein-protein, protein-nucleic acid, or protein-drug interactions, and the impact of metabolite binding and post-translational modifications on these interactions. The experiments quantitatively characterize biological samples treated with small molecules versus controls and subjected to timed exposures to multiple temperatures. Typically, each enzymatically digested sample is labeled with a tandem mass tag (TMT), where each TMT channel corresponds to a specific temperature treatment, and profiled using liquid chromatography coupled with mass spectrometry in data-dependent data acquisition mode. The resulting mass spectra are processed with computational tools to identify and quantify proteins and filter out noise. Protein-drug interactions are det

SUBMITTER: Figueroa-Navedo AM 

PROVIDER: S-EPMC12335990 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature