Bioluminescence-based assays for quantifying endogenous protein interactions in live cells.

ABSTRACT: Protein-protein interactions (PPIs) are integral to cellular signaling networks and are frequently disrupted in cancer, neurodegeneration, inflammation, and metabolic disorders. Targeting dysregulated PPIs presents a promising strategy for the development of therapeutic compounds. However, traditional drug discovery platforms often rely on plasmid-driven overexpression models that fail to replicate the complexity and dynamics of PPI in native cellular contexts. This study aims to evaluate the use of NanoLuc Binary Technology (NanoBiT) and NanoLuc Bioluminescence Resonance Energy Transfer (NanoBRET) for quantifying interactions of endogenously regulated proteins in live cells. To achieve this, CRISPR-mediated genome engineering was used to integrate NanoBiT and NanoBRET fusion tags at the l

SUBMITTER: Niles AL 

PROVIDER: S-EPMC12337185 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature