Cryo-EM structure and polar assembly of the PS2 S-layer of <i>Corynebacterium glutamicum</i>.

ABSTRACT: The polar-growing Corynebacteriales have a complex cell envelope architecture characterized by the presence of a specialized outer membrane composed of mycolic acids. In some Corynebacteriales, this mycomembrane is further supported by a proteinaceous surface layer or "S-layer," whose function, structure, and mode of assembly remain largely enigmatic. Here, we isolated ex vivo PS2 S-layers from the industrially important Corynebacterium glutamicum and determined its atomic structure by 3D cryo-EM reconstruction. PS2 monomers consist of a six-helix bundle "core," a three-helix bundle "arm," and a C-terminal transmembrane (TM) helix. The PS2 core oligomerizes into hexameric units anchored in the mycomembrane by a channel-like coiled-coil of the TM helices. The PS2 arms m

SUBMITTER: Sogues A 

PROVIDER: S-EPMC12337289 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature