SCoTCH-seq reveals that 5-hydroxymethylcytosine encodes regulatory information across DNA strands.

ABSTRACT: In mammalian genomes, cytosine modifications form a layer of regulatory information alongside the genetic code. Decoding this information is crucial to our understanding of biology and disease. Established sequencing methods cannot simultaneously resolve cytosine's three most common forms-cytosine (C), 5-methylcytosine (mC), and 5-hydroxymethylcytosine (hmC)-across both strands of the DNA double helix. Thus, how epigenetic information is distributed in DNA remains unclear. Here, we present Strand-Coupled Tandem Cytosine Hydroxymethylation and methylation sequencing (SCoTCH-seq): an accurate and quantitative, base-resolution approach to sequence genomes, together with mC and hmC, in both strands of the same DNA fragment. We show that different forms of cyt

SUBMITTER: Hardwick JS 

PROVIDER: S-EPMC12337322 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature