Study on <i>VEGFA</i> mRNA delivery via GelMA hydrogel-encapsulated extracellular vesicles for enhanced bone regeneration.

ABSTRACT: Bone regeneration remains a clinical challenge due to the inherent limitations of conventional grafts and synthetic materials. While mRNA-based strategies offer promising therapeutic potential, their clinical application is hindered by systemic delivery barriers and rapid degradation. Here, we present a hybrid extracellular vesicle (EV)-GelMA hydrogel system for the localized delivery of VEGFA mRNA to enhance both angiogenesis and osteogenesis through precise spatiotemporal control. In vitro, the VEGFA-EVs-GelMA system significantly enhanced migration and osteogenic differentiation of pre-osteoblastic cells, as evidenced by upregulation of ALP, RUNX2, and OPN expression compared to GelMA controls. In a rat cranial defect model, the VEGFA-EVs-GelMA group showed a marked increase in b

SUBMITTER: Li S 

PROVIDER: S-EPMC12337882 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature