Fortification of FeS Clusters Reshapes Anaerobic CO Dehydrogenase into an Air-Viable Enzyme Through Multilayered Sealing of O₂ Tunnels.

ABSTRACT: The inherent O₂ sensitivity of Ni─Fe carbon monoxide dehydrogenases (CODHs), crucial for rapid CO to CO₂ interconversion, presents substantial challenges for industrial application. Transforming CO/CO₂, a prevalent anthropogenic air pollutant, into valuable carbon chemicals either directly or through intermediate steps via biocatalytic methods offers a promising pathway to achieve net-zero emissions across industries and the environment. However, completely eliminating oxygen from industrial biotransformations, especially under ambient conditions, is exceedingly onerous. Here, we engineered variants of the CODH2 from Carboxydothermus hydrogenoformans (ChCODH2) with dual blocking at both the O₂ entrance and near the active site, effectively sealin

SUBMITTER: Kim SM 

PROVIDER: S-EPMC12338426 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature