Allosteric effects of the coupling cation in melibiose transporter MelB.

ABSTRACT: The major facilitator superfamily (MFS) transporters play significant roles in human health and disease. Salmonella enterica serovar Typhimurium melibiose permease (MelB_St), which catalyzes the symport of galactosides with Na⁺, H⁺, or Li⁺, is a prototype of this important transporter superfamily. We have published the structures of the inward- and outward-facing conformations of MelB_St with galactoside or Na⁺ bound, determined the binding thermodynamic cycle, and proposed that positive cooperativity between the two co-transported solutes plays a key role in the symport mechanism of MelB_St. The molecular basis for this core mechanism remains unclear. In this study, we determined the molecular basis for this

SUBMITTER: Hariharan P 

PROVIDER: S-EPMC12338509 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature