A multiplex extracellular interactome screening method employing high-avidity nanoparticles.

ABSTRACT: Metazoan cells signal to each other via direct contact between cell surface proteins (CSPs) and by interactions of CSP receptors with secreted ligands. CSP extracellular domain (ECD) interactions control organ development and physiology and are perturbed in disease states. However, because they cannot be accurately assessed using standard high-throughput screening techniques, they are underrepresented in protein interaction databases. Many ECD interactions are of low affinity, and their detection in vitro requires taking advantage of avidity effects, typically by multimerization of fusion proteins. Assays that test only one or a few interactions in each binding reaction are inadequate for global interactome screening. Here we describe a new multiplex method that uses purified dimeri

SUBMITTER: Anaya MA 

PROVIDER: S-EPMC12338595 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature