Endothelial Trauma Depends on Surface Charge and Extracellular Calcium Levels.

ABSTRACT: We hypothesized that cationic histones bind to endothelial cell membranes through electrostatic interactions with negatively charged phospholipids, and consequently, that low Ca ²⁺ exacerbates toxicity by increasing binding and uptake of histones into cells. The role of the ubiquitous store-operated Ca ²⁺ entry (SOCE) in histone responses is also unknown. Here, studying endothelial cells in surgical preparations and in culture, we observed clinically relevant histone concentrations, produced fast plasma membrane movements including vesiculation, blebbing, ruffling, and cellular collapse. The cell membrane theatrics observed were markedly different from the uniform pattern of exocytosis and blebbing produced by calcium overload with ionomycin. Interestingly, membrane p

SUBMITTER: Cleary JH 

PROVIDER: S-EPMC12338662 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature