Dermal fibroblast cultures recapitulate differences between deermice and mice in responses to a Toll-like receptor agonist.

ABSTRACT: The white-footed deermouse Peromyscus leucopus is a primary reservoir for the agents of Lyme disease and other zoonoses in North America and manifests infection tolerance for the bacteria, protozoa, and viruses it hosts. In previous in vivo studies P. leucopus and M. musculus differed in the degree of sickness and profiles of biomarkers after exposure to bacterial lipopolysaccharide, a TLR4 agonist. As an approach for assessing immunity of mammals in nature and for longitudinal studies of colony animals in the laboratory, we evaluated using bulk and single cell RNA-seq primary dermal fibroblast cultures of P. leucopus and M. musculus in their short-term responses to a TLR2 agonist lipopeptide. By single cell RNA-seq cultures of both species comprised at l

SUBMITTER: Duong JV 

PROVIDER: S-EPMC12338727 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature