NovoStoic2.0: An integrated framework for pathway synthesis, thermodynamic evaluation, and enzyme selection.

ABSTRACT: Computational pathway design and retro-biosynthetic approaches can facilitate the development of innovative biochemical production routes, biodegradation strategies, and the funneling of multiple precursors into a single bioproduct. However, effective pathway design necessitates a comprehensive understanding of biochemistries, enzyme activities, and thermodynamic feasibility. Herein, we introduce novoStoic2.0, an integrated platform that combines tools for estimating overall stoichiometry, designing de novo synthesis pathways, assessing thermodynamic feasibility, and selecting enzymes. novoStoic2.0 offers a unified web-based interface as a part of the AlphaSynthesis platform (http://novostoic.platform.moleculemaker.org/) tailored for the synthesis of thermodynamically viable pathways as we

SUBMITTER: Upadhyay V 

PROVIDER: S-EPMC12338773 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature