Hyperpolarized water as universal sensitivity booster in biomolecular NMR.

ABSTRACT: NMR spectroscopy is the only method to access the structural dynamics of biomolecules at high (atomistic) resolution in their native solution state. However, this method's low sensitivity has two important consequences: (i) typically experiments have to be performed at high concentrations that increase sensitivity but are not physiological, and (ii) signals have to be accumulated over long periods, complicating the determination of interaction kinetics on the order of seconds and impeding studies of unstable systems. Both limitations are of equal, fundamental relevance: non-native conditions are of limited pharmacological relevance, and the function of proteins, enzymes and nucleic acids often relies on their interaction kinetics. To overcome these limitations, we have developed applicatio

SUBMITTER: Hilty C 

PROVIDER: S-EPMC12339186 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature