Massively parallel immunopeptidome by DNA sequencing provides insights into cancer antigen presentation.

ABSTRACT: Human leukocyte antigens (HLAs) are encoded by the most polymorphic genes in the human genome. HLA class I alleles control antigen presentation for T cell recognition, which is pivotal for autoimmunity, infectious diseases and cancer. Current knowledge of HLA-bound peptides is limited, skewed and falls short of population-wide HLA binding profiles for high-value targets. Here we present ESCAPE-seq (enhanced single-chain antigen presentation sequencing), a massively parallel platform for comprehensive screening of class I HLA-peptide combinations for antigen presentation via deep DNA sequencing. ESCAPE-seq demonstrates programmability, high throughput, sensitivity and nominated viral and cancer epitopes. We simultaneously assessed over 75,000 peptide-HLA combinations, revealing broadly pres

SUBMITTER: Shi Q 

PROVIDER: S-EPMC12339365 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature