Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells.

ABSTRACT: Hematopoietic stem cells (HSCs) derived from human induced pluripotent stem cells (iPS cells) have important biomedical applications. We identified differentiation conditions that generate HSCs defined by robust long-term multilineage engraftment in immune-deficient NOD,B6.Prkdc^scid Il2rg^tm1Wjl/SzJ Kit^W41/W41 mice. We guided differentiating iPS cells, as embryoid bodies in a defined culture medium supplemented with retinyl acetate, through HOXA-patterned mesoderm to hemogenic endothelium specified by bone morphogenetic protein 4 and vascular endothelial growth factor (VEGF). Removal of VEGF facilitated an efficient endothelial-to-hematopoietic transition, evidenced by release into the culture medium of CD34⁺ blood cells, which were cryopreserved.

SUBMITTER: Ng ES 

PROVIDER: S-EPMC12339382 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature