Computational approach involving use of the internal transcribed spacer 1 region for identification of Mycobacterium species.

ABSTRACT: The rapid and reliable identification of clinically significant Mycobacterium species is a challenge for diagnostic laboratories. This study evaluates a unique sequence-dependent identification algorithm called MycoAlign for the differential identification of Mycobacterium species. The MycoAlign system uses pan-Mycobacterium-specific primer amplification in combination with a customized database and algorithm. The results of testing were compared with conventional phenotypic assays and GenBank sequence comparisons using the 16S rRNA target. Discrepant results were retested and evaluated using a third independent database. The custom database was generated using the hypervariable sequences of the internal transcribed spacer 1 (ITS-1) region of the rRNA gene complex from characterized Mycoba

SUBMITTER: Mohamed AM 

PROVIDER: S-EPMC1233978 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature