Synergistic targeting of cancer-related proteins by benzyl isothiocyanate and caffeic acid: MLSD and cytotoxic mechanisms in MCF-7 cells.

ABSTRACT: This study investigated the synergistic cytotoxic effects of benzyl isothiocyanate (BITC) and caffeic acid (CA) on human breast adenocarcinoma (MCF-7) cells. Cell viability assays (MTT) revealed that BITC induces reactive oxygen species (ROS)-mediated cell death, while CA alone exhibited minimal cytotoxicity. Notably, their combination selectively enhanced MCF-7 cell death with limited effects on human fibroblasts. Mechanistic investigations demonstrated MAPK pathway activation and apoptosis induction, supported by altered expression of GST, p38, p-ERK, ERK 1/2, Nrf-2, and Bcl-2 proteins. Fluorometric analysis revealed significant disruption of redox homeostasis, including changes in ROS, glutathione (GSH), and caspase-3/7 activity. Molecular docking studies confirmed stable binding intera

SUBMITTER: Rahaman MHA 

PROVIDER: S-EPMC12339810 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature