Comparative effects of standardized Centella asiatica extract (ECa 233) and its active compound mixture on proteomics and mitochondrial function.

ABSTRACT: ECa 233 is a standardized extract of Centella asiatica that contains the major active compounds madecassoside (MDS) and asiaticoside (ASS), which can strengthen hippocampal synapses, resulting in a memory-enhancing effect. This study explored the possible mechanisms of ECa 233 related to synaptic enhancement via proteomic analysis. Differentiated SH-SY5Y cells and hippocampal slices obtained from healthy male Wistar rats were treated with dimethyl sulfoxide, ECa 233, or the MDS + ASS mixture. Mitochondrial function and protein expression were examined via proteomic, western blot, and immunofluorescence analyses. Effects on synaptic plasticity were confirmed via electrophysiology using acute hippocampal slices. The treated groups exhibited a significant increase in mitochondrial activity an

SUBMITTER: Boondam Y 

PROVIDER: S-EPMC12339980 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature