The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in <i>Vibrio parahaemolyticus</i>.

ABSTRACT: Vibrio parahaemolyticus is a zoonotic pathogen that poses a serious threat to shrimp aquaculture and public health worldwide. Copper (Cu) serves as a catalytic cofactor and structural element in bacteria but is toxic in excess. Herein, we report that the CueR-regulated transporters CopA and CusFABC coordinate Cu detoxification in V. parahaemolyticus. Through RNA sequencing, we show that most genes in the CueR and Fur regulons are significantly upregulated during V. parahaemolyticus response to external Cu. Quantitative real-time PCR (qRT-PCR) showed that copA and cusA are strongly induced by Cu. Growth curve analyses and spot dilution assay revealed that CopA and CusFABC protect V. parahaemolyticus against Cu-induced bactericidal effects. Inductive

SUBMITTER: Zheng C 

PROVIDER: S-EPMC12341060 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature