Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex.

ABSTRACT: A single multilocus sequence typing (MLST) scheme was developed for precise characterization of the opportunistic pathogens of Burkholderia cepacia complex (BCC), a group composed of at least nine closely related species. Seven conserved housekeeping genes were selected after a comparison of five Burkholderia species, and a collection of strains was subjected to nucleotide sequence analysis using a nested PCR amplification approach for each gene. MLST differentiated all nine current BCC species and identified 114 sequence types within a collection of 119 strains. No differentiation was found between strains recovered from environmental or clinical sources. The improved resolution in strain identification offered by MLST was able to identify previously characterized epidemic strain lineages

SUBMITTER: Baldwin A 

PROVIDER: S-EPMC1234123 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature