Isopropanol production from carbon dioxide by <i>Cupriavidus necator</i> using a zero-gap cell with culture broth as catholyte.

ABSTRACT: Microbial CO₂ fixation is a promising route for sustainable chemical production. The facultative chemolithoautotroph Cupriavidus necator can convert CO₂ into value-added compounds using H₂ and O₂. Here, we developed a bioelectrochemical system (BES) for isopropanol production that integrates CO₂ fixation with in situ H₂ generation via a zero-gap proton exchange membrane electrolyzer. Using the culture broth as catholyte, a pentlandite-based cathode supplied dissolved H₂ directly to engineered C. necator, eliminating the need for pressurized gas. Real-time monitoring of dissolved and off-gas compositions guided system operation, yielding an isopropanol titer of 1.1 g L^-1. The pentlan

SUBMITTER: Schoenmakers P 

PROVIDER: S-EPMC12341536 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature