Massive field-of-view sub-cellular traction force videography enabled by Single-Pixel Optical Tracers (SPOT).

ABSTRACT: We report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm² at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction force into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral

SUBMITTER: Marvin Tan XH 

PROVIDER: S-EPMC12341656 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature