Microglia Single-Cell RNA-Seq Enables Robust and Applicable Markers of Biological Aging.

ABSTRACT: "Biological aging clocks"-composite molecular markers thought to capture an individual's biological age-have been traditionally developed through bulk-level analyses of mixed cells and tissues. However, recent evidence highlights the importance of gaining single-cell-level insights into the aging process. Microglia are key immune cells in the brain shown to adapt functionally in aging and disease. Recent studies have generated single-cell RNA-sequencing (scRNA-seq) datasets that transcriptionally profile microglia during aging and development. Leveraging such datasets in humans and mice, we develop and compare computational approaches for generating transcriptome-wide summaries from microglia to establish robust and applicable aging clocks. Our results reveal that unsupervised, frequency-b

SUBMITTER: Stanley N 

PROVIDER: S-EPMC12341818 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature