Using repeated lysis steps fractionates between heterotrophic and cyanobacterial DNA extracted from xenic cyanobacterial cultures.

ABSTRACT: Extracting DNA from cyanobacteria can be a challenge because of their diverse morphologies, challenging cellular structure, and the heterotrophic microbiome often present within cyanobacterial cultures. As such, even with high DNA yields, the percentage of reads coming from the cyanobacterial host can be low, leading to an incomplete cyanobacterial genome assembly. In this research, we optimized a DNA isolation protocol using three iterative cell lysis steps to enrich the portion of DNA isolated coming from the cyanobacterial host rather than the heterotrophic microbiome. In order to utilize in-house nanopore sequencing, we faced a challenge using our lysis protocol: the iterative lysis approach led to more DNA shearing than is ideal for this sequencing technology. To solve this, we used t

SUBMITTER: Wagner AD 

PROVIDER: S-EPMC12341861 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature