Use of sequence barcodes for tracking horizontal gene transfer of antimicrobial resistance genes in a microbial community.

ABSTRACT: One of the most important knowledge gaps in the antimicrobial resistance crisis is the lack of understanding regarding how genes spread from their environmental origins to bacteria pathogenic to humans. In this study our aim was to create a system that allows the conduction of experiments in laboratory settings that mimic the complexity of natural communities with multiple resistance genes and mobile genetic elements circulating at the same time. Here we report a new sequence-based barcode system that allows simultaneous tracking of the spread of antimicrobial resistance genes from multiple genetic origins. We tested this concept with an experiment in which we added an antimicrobial resistance gene to different genetic environments in alive and dead donors and let the gene spread naturally

SUBMITTER: Partanen V 

PROVIDER: S-EPMC12343072 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature