Diminished immune cell adhesion in hypoimmune ICAM-1 knockout human pluripotent stem cells.

ABSTRACT: Gene edited human pluripotent stem cells are a promising platform for developing reparative cellular therapies that evade immune rejection. Existing first-generation hypoimmune strategies have used CRISPR/Cas9 editing to modulate genes associated with adaptive immune responses, but have largely not addressed the innate immune cells, such as neutrophils, that mediate inflammation and rejection processes occurring early after graft transplantation. We identify the adhesion molecule ICAM-1 as a hypoimmune target that plays multiple critical roles in both adaptive and innate immune responses post-transplantation. In our experiments, we find that ICAM-1 blocking or knockout in human pluripotent stem cell-derived cardiovascular therapies imparts significantly diminished binding of multiple immun

SUBMITTER: Saha S 

PROVIDER: S-EPMC12343971 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature