Maturation of Human iPSC-Derived Cardiac Microfiber with Electrical Stimulation Device.

ABSTRACT: Here an electrical stimulation system is described for maturing microfiber-shaped cardiac tissue (cardiac microfibers, CMFs). The system enables stable culturing of CMFs with electrical stimulation by placing the tissue between electrodes. The electrical stimulation device provides an electric field covering whole CMFs within the stimulation area and can control the beating of the cardiac microfibers. In addition, CMFs under electrical stimulation with different frequencies are examined to evaluate the maturation levels by their sarcomere lengths, electrophysiological characteristics, and gene expression. Sarcomere elongation (14% increase compared to control) is observed at day 10, and a significant upregulation of electrodynamic properties such as gap junction protein alpha 1 (GJA1) and

SUBMITTER: Masuda A 

PROVIDER: S-EPMC12344631 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature