RNA Binding Mechanism of the FUS Zinc Finger in Concert with Its Flanking Intrinsically Disordered Region.

ABSTRACT: The zinc finger (ZnF) motif is one of the most common nucleic acid binding motifs in proteins and comprises approximately 5% of the human genome. Proteins often carry multiple copies of ZnF motifs, enhancing DNA- and RNA-binding affinity and specificity. Fused in Sarcoma (FUS) is an RNA-binding protein that contains one ZnF motif and one RNA recognition motif. We identified the molecular basis for the strong and specific binding of FUS ZnF to RNA at atomic resolution by performing molecular dynamics and dissociation parallel cascade selection molecular dynamics simulations of a single-stranded RNA complexed with FUS ZnF and the flanking RGG2 domain. The RGG2-ZnF construct was classified into two regions based on atomic fluctuation: an intrinsically disordered region (IDR) consisting mainly

SUBMITTER: Kijima S 

PROVIDER: S-EPMC12344763 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature