Comparative assessment of line probe assays and targeted next-generation sequencing in drug-resistant tuberculosis diagnosis.

ABSTRACT:

Background

Rapid and accurate detection of drug-resistant tuberculosis (DR-TB) is crucial for ensuring effective treatment, halting transmission and preventing the amplification of resistance. Comparative evaluations of molecular diagnostic assays in high-burden settings are essential for informing clinical decision-making for DR-TB treatment.

Methods

The Seq&Treat clinical study previously evaluated the performance of two targeted next-generation sequencing (tNGS) workflows, GenoScreen Deeplex Myc-TB and Oxford Nanopore Technologies Tuberculosis Drug Resistance Test, on direct sediment samples from persons at risk for DR-TB. Hain Line Probe Assay (LPAs-MTBDRplus and MTBDRsl) were run as a comparator test using an aliquot of the same sediment samples. Diagnostic performance

SUBMITTER: Carpi G 

PROVIDER: S-EPMC12344966 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature