Development of selective culture media for efficient isolation of <i>Avibacterium paragallinarum</i> from chickens.

ABSTRACT: Infectious coryza (IC) caused by Avibacterium paragallinarum (AvP) is an upper respiratory disease in chickens and incurs a significant economic impact on laying hens. Control of IC requires reliable bacterial isolation, but AvP is a fastidious bacterium, and the currently used methods yield poor isolation. To address this need, we developed selective media for the efficient growth and isolation of AvP. Several basal media supplemented with various growth factors were explored, and Mueller Hinton agar (MHA) supplemented with fetal bovine serum (FBS) plus NAD yielded optimal AvP growth, eliminating the need for nurse bacteria. This medium (named MSN) was further supplemented with vancomycin and crystal violet to produce two selective media, named MSNV and MSCV, respectively, to inhib

SUBMITTER: Srednik ME 

PROVIDER: S-EPMC12345139 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature