Histone variant H2A.Z cooperates with EBNA1 to maintain Epstein-Barr virus latent epigenome.

ABSTRACT: Chromatin structure plays a central role in the regulation of Epstein-Barr virus (EBV) latency. The histone variant H2A.Z.1 has been implicated in chromatin structures associated with the initiation of transcription and DNA replication. Here, we investigate the functional role of H2AZ.1 in the regulation of EBV chromatin, gene expression, and copy number during latent infection. We found that H2A.Z.1 is highly enriched near EBNA1-binding sites at the origin of plasmid replication (oriP) and the transcriptional start site for the EBNA1 gene (Qp), and to a lesser extent with transcriptionally active CTCF binding sites on the EBV genomes in both Mutu I Burkitt lymphoma (BL) and SNU719 EBV-associated gastric carcinoma (EBVaGC) cell lines. RNA-interference depletion of H2A.Z.1 resulted i

SUBMITTER: Castro-Munoz LJ 

PROVIDER: S-EPMC12345143 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature