Hybridization capture sequencing for <i>Vibrio</i> spp. and associated virulence factors.

ABSTRACT: Proliferation of Vibrio spp. in aquatic ecosystems is associated with climate change and, concomitantly, increased incidence of vibriosis. They are autochthonous to aquatic environments globally, but traditional metagenomic methods for detecting and typing pathogenic Vibrio spp. are challenged by their presence in relatively low abundance and ability to persist in a viable but nonculturable state. In the study reported here, hybridization capture sequencing (HCS) was employed to profile low-abundance Vibrio spp. in environmental samples. The HCS panel targeted a family of molecular chaperones (CPN60) specific to 69 Vibrio spp. and 162 Vibrio-specific virulence factors. This approach was evaluated in parallel with traditional whole-community shotgun sequen

SUBMITTER: Brumfield KD 

PROVIDER: S-EPMC12345146 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature