Macrophage phagocytosis of human norovirus-infected cells in an <i>ex vivo</i> human enteroid-macrophage coculture model.

ABSTRACT: Human norovirus (HuNoV) causes acute gastroenteritis in immunocompetent hosts and chronic infection in immunocompromised individuals. Many recent studies of replication and innate immune responses following HuNoV infection have utilized epithelium-only human intestinal enteroids (HIEs), which lack immune cells. Here, we utilized an ex vivo enteroid-macrophage coculture model consisting of HIEs and different subtypes of human peripheral blood mononuclear cell-derived macrophages to better recapitulate in vivo gut biology and explore the role of macrophages in HuNoV replication and pathogenesis. We show that HuNoV infection in HIEs polarized on Transwells leads to bilateral release of the viral genome, with predominant apical virus release. Coculture with naïve M0, pro-inflamma

SUBMITTER: Li NF 

PROVIDER: S-EPMC12345152 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature