An ELISA-like sensitive and visual detection system targeting <i>Yersinia pestis</i> based on CRISPR/Cas12a and DNAzyme.

ABSTRACT: Yersinia pestis is the causative agent of plague, a human disease with potentially devastating consequences. Here, we developed an enzyme-linked immunosorbent assay-like visual detection method based on clustered regularly interspaced short palindromic repeats (CRISPR) detection and DNAzyme for the cost-effective and highly sensitive detection of Y. pestis. A novel specific gene sequence (CH57_3927) was screened for the detection target of Y. pestis. The recombinase-aided amplification (RAA) assay, CRISPR/Cas12a detection assay, and G-quadruplex (G4) DNAzyme-based color development assay were separately established and optimized. These three optimized assays were integrated into an advanced ELISA-like visual detection method-RAA-CRISPR/Cas12a-DNAzyme (RCCD)-by further

SUBMITTER: Mao Y 

PROVIDER: S-EPMC12345247 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature