TGF-β1 Inhibits Osteogenesis via ZBTB16 Regulation in Periodontal Ligament.

ABSTRACT:

Objective

To elucidate pivotal molecules through which transforming growth factor-β1 (TGF-β1) regulates osteogenesis in human periodontal ligament (PDL) cells.

Methods

In vitro, human PDL, pulpal, and gingival cells were obtained from healthy premolars that were extracted for orthodontic treatment. Cells were treated with dexamethasone for differentiation and alkaline phosphatase and alizarin red staining were used for the mineralization assay. In vivo, the upper first molars in rats were mesially moved. Genes were identified by RNA sequencing and bioinformatics analysis. The expression levels and histological localization of TGF-β1 and ZBTB16 were determined by real-time RT-PCR and Western blotting, and immunofluorescence, respectively.

Results

RNA sequencing identif

SUBMITTER: Moon JS 

PROVIDER: S-EPMC12345258 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature