Fourier synthesis optical diffraction tomography for kilohertz rate volumetric imaging.

ABSTRACT: Many biological and soft matter processes occur at high speeds in complex three-dimensional (3D) environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding challenge. Here, we introduce Fourier synthesis optical diffraction tomography (FS-ODT), a quantitative phase imaging approach capable of recording the 3D refractive index at kilohertz rates. FS-ODT introduces computational strategies that multiplex tens of illumination angles in a single tomogram, markedly increasing the volumetric imaging rate. We validate FS-ODT on samples of known composition, hindered diffusion of colloids in solution, and the motility of bacterial swimmers. We also integrate FS-ODT into a multimodal microscope combining refractive index imaging with multicolor structure

SUBMITTER: Brown PT 

PROVIDER: S-EPMC12346287 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature