Human Omental Mesothelial Cells Exhibit a Corneal Endothelial-Like Cell Phenotype for Tissue Engineering of a Corneal Endothelium Biomimetic.

ABSTRACT:

Purpose

To evaluate whether cultured human omental mesothelial cells (OMC) exhibit phenotypic and functional similarities to human corneal endothelial cells (CEC) and whether they can adhere to the corneal stroma and form a biomimetic corneal endothelium when grown on a human anterior lens capsule (HALC).

Methods

Human OMC were isolated from the greater omentum. Human B4G12 CEC were used as a reference for native and functional CEC phenotype whereas human mesenchymal stromal cells (MSC) served as a phenotypically distinct control group. OMC, CEC and MSC were compared using flow cytometry, immunofluorescence and qRT-PCR for lineage-specific markers, as well as through a transepithelial permeability assay. The adhesion capacity of OMC was also evaluated on both mouse corneal s

SUBMITTER: Alio JL 

PROVIDER: S-EPMC12347251 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature