Brain-wide neuronal circuit connectome of human glioblastoma.

ABSTRACT: Glioblastoma (GBM) infiltrates the brain and can be synaptically innervated by neurons, which drives tumour progression^1,2. Synaptic inputs onto GBM cells identified so far are largely short range and glutamatergic^3,4. The extent of GBM integration into the brain-wide neuronal circuitry remains unclear. Here we applied rabies virus-mediated and herpes simplex virus-mediated trans-monosynaptic tracing^5,6 to systematically investigate circuit integration of human GBM organoids transplanted into adult mice. We found that GBM cells from multiple patients rapidly integrate into diverse local and long-range neural circuits across the brain. Beyond glutamatergic inputs, we identified various neuromodulatory inputs, including synapses between basal forebrain cholin

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12347542 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature