Transcriptional Repression of CCL2 by K_Ca3.1 K⁺ Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M₂ Macrophages.

ABSTRACT: We investigated the role of the intermediate-conductance, Ca²⁺-activated K⁺ channel K_Ca3.1 and volume-regulatory anion channel LRRC8A in regulating C-C motif chemokine ligand 2 (CCL2) expression in THP-1-differentiated M₂ macrophages (M₂-MACs), which serve as a useful model for studying tumor-associated macrophages (TAMs). CCL2 is a potent chemoattractant involved in the recruitment of immunosuppressive cells and its expression is regulated through intracellular signaling pathways such as ERK, JNK, and Nrf2 in various types of cells including macrophages. The transcriptional expression of CCL2 was suppressed in M₂-MACs following treatment with a K_Ca3.1 activator or an LRRC8A inhibitor via distinct signaling pathwa

SUBMITTER: Matsui M 

PROVIDER: S-EPMC12347553 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature