Enhancement of D-mannitol production by fine-tuned expression of mannitol-1-phosphate dehydrogenase in Synechocystis sp. PCC6803.

ABSTRACT: D-Mannitol production was achieved in freshwater Synechocystis sp. PCC6803 via the heterologous expression of mannitol-1-phosphate dehydrogenase (mtlD) and mannitol-1-phosphatase (m1p) under control of the strong promoter P_trc1. However, only 5.54 mg L^-1 of mannitol was found extracellularly after 7 days of cultivation, likely due to insufficient expression of a mutated mtlD lacking a methionine at position 332. This study compared mannitol levels using different promoters (P_trc1, P_psbA2 and P_nrsB) to control the expression of (un)mutated versions of mtlD in Synechocystis with co-expression of m1p. Our data suggest that even without the inducer, the weakest promoter, P_nrsB, can support the expression of an unmutated mtlD in

SUBMITTER: Wu W 

PROVIDER: S-EPMC12348302 | biostudies-literature | 2025 Jul-Aug

REPOSITORIES: biostudies-literature