Prevotella intermedia Synergistically Exacerbates Pneumonia Induced by Oral Streptococci.

ABSTRACT:

Background

The precise mechanisms of respiratory infection caused by oral anaerobic bacteria remain elusive. Unexpectedly, bacterial microbiota analysis with 16S rRNA revealed "hidden" mixed infections of anaerobic bacteria and commensal oral Streptococcus species in patients with community-acquired pneumonia. The study aimed to elucidate the mechanisms by which Prevotella intermedia exacerbates oral streptococcal pneumonia.

Methods

Oral streptococci were oropharyngeally administered with the culture supernatants of P intermedia (PiSup) in mice to assess survival, microbial burden, inflammatory responses, and host response via unbiased bulk RNA sequencing. Additionally, genetically engineered strains of P intermedia were used to identify pathogenic factors.

Results

Se

SUBMITTER: Ashizawa H 

PROVIDER: S-EPMC12349947 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature