MALAT1 regulates mRNA processing through sequence dependent RNA-RNA and RNA-protein interactions.

ABSTRACT: Messenger RNAs (mRNAs) are subject to multiple layers of gene expression regulation, enabling the production of a large diversity of RNA transcripts and encoded proteins from a smaller number of genes in the human genome. We find that the metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) non-coding RNA regulates mRNA processing through direct RNA-RNA and RNA-protein interactions. MALAT1 interacts with both the TAR DNA binding protein (TDP-43) and the spermine/spermidine acetyltransferase SAT1 pre-mRNA to enhance alternative splicing of SAT1 through direct, sequence-specific interactions. MALAT1 interaction with TDP-43 and SAT1 increases TDP-43 binding affinity for SAT1 pre-mRNA by coordinating tripartite RNA-RNA-protein interactions. These tripartite interactions enhance SAT1

SUBMITTER: Balaji A 

PROVIDER: S-EPMC12350098 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature