Pathology-oriented multiplexing enables integrative disease mapping.

ABSTRACT: The expression and location of proteins in tissues represent key determinants of health and disease. Although recent advances in multiplexed imaging have expanded the number of spatially accessible proteins^1-3, the integration of biological layers (that is, cell structure, subcellular domains and signalling activity) remains challenging. This is due to limitations in the compositions of antibody panels and image resolution, which together restrict the scope of image analysis. Here we present pathology-oriented multiplexing (PathoPlex), a scalable, quality-controlled and interpretable framework. It combines highly multiplexed imaging at subcellular resolution with a software package to extract and interpret protein co-expression patterns (clusters) across biological layers. Patho

SUBMITTER: Kuehl M 

PROVIDER: S-EPMC12350167 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature