Mapping and engineering RNA-driven architecture of the multiphase nucleolus.

ABSTRACT: Biomolecular condensates are key features of intracellular compartmentalization^1,2. As the most prominent nuclear condensate in eukaryotes, the nucleolus is a multiphase liquid-like structure in which ribosomal RNAs (rRNAs) are transcribed and processed, undergoing multiple maturation steps to form the small (SSU) and large (LSU) ribosomal subunits^3-5. However, how rRNA processing is coupled to the layered organization of the nucleolus is poorly understood owing to a lack of tools to precisely monitor and perturb nucleolar rRNA processing dynamics. Here we developed two complementary approaches to spatiotemporally map rRNA processing and engineer de novo nucleoli. Using sequencing in parallel with imaging, we found that rRNA processing steps are spatially segregated,

SUBMITTER: Quinodoz SA 

PROVIDER: S-EPMC12350172 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature