Mouse liver assembloids model periportal architecture and biliary fibrosis.

ABSTRACT: Modelling liver disease in vitro requires systems that replicate disease progression^1,2. Current tissue-derived organoids do not reproduce the complex cellular composition and tissue architecture observed in vivo³. Here, we describe a multicellular organoid system composed of adult hepatocytes, cholangiocytes and mesenchymal cells that recapitulates the architecture of the liver periportal region and, when manipulated, models aspects of cholestatic injury and biliary fibrosis. We first generate reproducible hepatocyte organoids with a functional bile canaliculi network that retain morphological features of in vivo tissue. By combining these with cholangiocytes and portal fibroblasts, we generate assembloids that mimic the cellular interactions of the periportal region

SUBMITTER: Dowbaj AM 

PROVIDER: S-EPMC12350178 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature