Anion exchange chromatography-based purification of plant-derived nanovesicles from <i>Brassica oleracea</i> L.: molecular profiling and bioactivity in human cells.

ABSTRACT: Plant-derived nanovesicles emerge as a promising alternative to mammalian-derived exosomes with distinct advantages, including lower immunogenicity, enhanced bioavailability, and the presence of bioactive plant metabolites. They have been shown to cross biological barriers, delivering therapeutic molecules that modulate gene expression, inflammation, oxidative stress, and cancer-related pathways. However, challenges remain that limit applicative use, including poor knowledge of their interactions with mammalian host cells and primarily the lack of a cost-effective and scalable method to obtain highly purified plant-derived nanovesicles. To address these limitations, we have developed an advanced purification platform that integrates ultrafiltration with anion exchange chromatography in a f

SUBMITTER: Zanotti C 

PROVIDER: S-EPMC12350333 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature