Serum profiling of the antibody response to HPV in women with or without abnormal cervical cytology undergoing cervical cancer screening.

ABSTRACT:

Introduction

Understanding the humoral immune response to HPV is important for understanding the natural history of infection and developing biomarkers for early detection of cervical cancer. This has been technically limited by HPV type diversity and challenges of high-throughput protein expression and display. This study aimed to profile the humoral immune response to the proteomes of 12 HPV types in women with or without abnormal cervical cytology undergoing cervical cancer screening.

Methods

To detect serum antibodies (Abs) against HPV, we developed custom HPV high-density diffusion-free nucleic acid programmable protein arrays (HD-NAPPA) displaying the proteomes of 2 low-risk (HPV6 and 11) and 10 high-risk (HR) HPV types (HPV16, 18, 31, 33, 35, 39, 45, 51, 52 and 58). A

SUBMITTER: Ewaisha R 

PROVIDER: S-EPMC12350350 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature